The self-proclaimed 'Baddest Man on the Planet' and 'The Problem Child' locked eyes at the weigh-in before Tyson gave Jake an early hit when he smacked him across the face at the weigh-in at The Pavilion at Toyota Music Factory, in Irving, Texas.
After security jumped in, Jake, 27, hit back at his "cute slap" by warning Mike, 58, he is "getting knocked the f*** out" in their boxing match on Friday (15.11.24).
Following the heated exchange on Thursday (14.11.24) ahead of the fight at the AT+T Stadium, in Arlington, Texas, Tyson revealed he slapped his opponent because he stepped on his feet and he had no shoes on.
Speaking to The New York Post newspaper, he said: "I was in my socks and he had on shoes. He stepped on my toe because he is a f***ing a**hole. I wanted to think it happened by accident.
"But now I think it may have happened on purpose. I was in a lot of pain. I had to reciprocate."
Ahead of the fight, which starts at 10pm local time, the pair carried out a weigh-in.
Mike - who is returning to the ring after a 19-year absence - came in at 227.2 pounds, with Jake coming a little bit heavier at 228.4 pounds.
'Jake Paul vs. Mike Tyson' will be available to stream live on Netflix.